Genetic Characterization of Field Isolates of Maedi-visna Virus (mvv) in Sheep from Poland
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چکیده
The 375 bp fragment of gag gene encoding fragment of matrix protein p16 of ten field isolates of maedi-visna virus (MVV) was amplified; and all the sequences were subsequently subjected to sequencing and genetic analysis. Among isolates, the homologies ranged from 87.2% to 100% and 88% to 100% at the nucleotide and amino acid level, respectively. Polish strains were more related to ovine prototypic strains with average variability of 21% in nucleotide sequence, whereas in comparison to the caprine strain CAEVCO, 33% divergence was demonstrated. Phylogenetic analysis enabled the segregation of the Polish strains into two major groups, closely related to MVVK1514 and two other ovine strains: SAOMVV and MVVEV1, confirming the presence of a complex population of MVV genotypes found in sheep from Poland.
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تاریخ انتشار 2006